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15(6): 639656 Detection of Mycotoxins in Patients with Chronic Fatigue Syndrome

In contrast, 2-AR is less abundant and primarily localized to hepatocytes, where it modulates oxidative stress responses 20

After denaturing in a boiling water bath, the proteins were separated by SDSpolyacrylamide gel electrophoresis (SDSPAGE), transferred to a PVDF membrane, blocked with 5% milk in TBST for 30 min at room temperature, and then incubated with primary antibody at 4 C overnight with shaking